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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become observed in several tumors, which results in chromosome instability, and therefore may lead to tumorigenesis. This protein was found to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a crucial position in promoting cell-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, are explained. Two further splice variants ended up reported but thorough nucleotide sequence information and facts just isn't still available.
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Blocking peptides are peptides that bind specially for the concentrate on antibody and block antibody binding. These peptide normally consists of the epitope identified by the antibody.
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This manual outlines more feasible optimization of cellular and immunoassay parameters to be sure the absolute best results are...
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This information outlines more possible optimization of mobile and immunoassay parameters to be certain the best possible results are...
Antibodies certain to the blocking peptide no longer bind to your epitope within the focus on protein. By comparing the staining in the blocked antibody vs . the antibody by yourself, one can see which staining is particular.
Focus on Details Cyclin E belongs to the highly conserved cyclin family, whose associates are characterized by a remarkable periodicity in protein abundance through the mobile cycle. Cyclins perform as regulators of CDK kinases. Unique cyclins exhibit distinctive expression and degradation designs which add towards the temporal coordination of each and every mitotic occasion. Cyclin E varieties a complex with and features like a regulatory subunit of CDK2, whose action is necessary for cell cycle G1/S transition. This protein accumulates for the G1-S phase boundary and is also degraded as cells development through S stage.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by each antibodies...
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